Journal: Oncotarget

Article Title: Magnolol and honokiol exert a synergistic anti-tumor effect through autophagy and apoptosis in human glioblastomas

doi: 10.18632/oncotarget.8674

Figure Lengend Snippet: LN229 and U87MG cells were treated with different concentrations of Hono, Mag or Hono-Mag for 24 hours. Protein expression levels of ( A ) LN229 and ( B ) U87MG were analyzed for cyclin A, cyclin D1, CDK2, CDK4 and CDK6 by Western blotting. GAPDH was used as an internal control. The panels show the quantitative analyses. * p < 0.05; ** p < 0.01; *** p < 0.001 compared to the control group. # p < 0.05; ## p < 0.01; ### p < 0.001 compared to the same concentration of Hono-treated group. ▲ p < 0.05; ▲▲ p < 0.01; ▲▲▲ p < 0.001 compared to the same concentration of Mag-treated group.

Article Snippet: Strips from the membrane were blocked with 5% non-fat milk in Tris-buffered saline, pH 8.2, containing 0.1% Tween (TBS-Tween) and incubated overnight at 4°C with a 1:500 dilution of rabbit antibodies against phosphorylated phosphoinositide 3-kinase (p-PI3K), cyclin-dependent kinase 2 (CDK2), CDK4, CDK6, phosphorylated JNK (p-JNK), JNK, phosphorylated ERK (p-ERK), ERK, phosphorylated p38 (p-p38), p38, Poly ADP ribose polymerase (PARP), cleaved caspase-3, cleaved caspase-8, cleaved caspase-9 (Cell Signaling), phosphorylated Akt (p-Akt) (Epitomics), Akt, cyclin A, cyclin D1 (Abcam), Bcl-2, GAPDH (BioVision), and Ki-67 (Genetex).

Techniques: Expressing, Western Blot, Concentration Assay